WebYou have just purified plasmid DNA from a liquidE. colicell culture by the DNA Miniprep method. In order to assess the purity and yield of DNA, you take a spectrophotometric measurement. After diluting 20 µL of the DNA to a final volume of 1000 µL, you find that the absorbance at 260 nm is 0.135 and the absorbance at 280 nm is 0.073. WebMolecular Formula: C 12H 15NO 8 Molecular Weight: 301.3 CAS Number: 369-07-3 Melting Point: 193-194 °C 1 Specific Rotation: -67° (1 g/100 ml water at 25 °C) Synonym: ONPG is prepared in 100 mM sodium phosphate buffer, pH This product (ONPG) is a colorimetric substrate for 7.3. The solution is gently warmed to complet β-galactosidase.
BIOL 111 Lab Flashcards Quizlet
Webβ-Galactosidase (EC 3.2.1.23, lactase, beta-gal or β-gal; systematic name β-D-galactoside galactohydrolase), is a glycoside hydrolase enzyme that catalyzes hydrolysis of terminal non-reducing β-D-galactose residues in … WebThis will give you an answer in mL (0.05 mL), but we are using μL, so you will need to convert: 0.05 mL (1000 μL/1 mL) = 50 μL. Calculate how much Z buffer. The volume of … greens heating and cooling
[ONPG] 2-Nitrophenyl-β-D-Galactopyranoside, 1 Gram
Web1 de jan. de 2001 · Beta-Galactosidase [EC 3.2.1.23] has been purified from a culture of Aspergillus oryzae by 2-propanol fractionation, column chromatography on DEAE … WebONPG ELISA Beta-Galactosidase Substrate is a useful detection for ELISA. Purity/Specificity. Purity: >95% Melting Range: 174° to 177° C Molecular Weight: 301.25 Formula: C12H15NO8 Grade: UltaPure Special Precautions: Use good judgement and appropriate handling procedures when weighing. Web1. Graph the results of each experiment. Make sure to title your graph and label the axes. The y-axis will be activity and the x-axis will be either substrate concentration or enzyme volume.Do not fit the plot to a line or perform regression analysis. GRAPHS LOCATED AFTER QUESTION 5 2. The molecular weight of ONPG is 301 g/mol. Calculate the … green sheath dress with jacket